human colon cancer cells adherent atcc Search Results


hct116  (ATCC)
99
ATCC hct116
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Hct116, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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125i-gm-csf 118 mci/mg  (DuPont de Nemours)
90
DuPont de Nemours 125i-gm-csf 118 mci/mg
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
125i Gm Csf 118 Mci/Mg, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant gm-csf  (Genzyme)
90
Genzyme human recombinant gm-csf
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Human Recombinant Gm Csf, supplied by Genzyme, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human colon carcinoma cells  (ATCC)
99
ATCC human colon carcinoma cells
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Human Colon Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gm-csf cytokine  (Sandoz)
90
Sandoz gm-csf cytokine
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Gm Csf Cytokine, supplied by Sandoz, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human gm csf  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti human gm csf
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Anti Human Gm Csf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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colonic adenocarcinoma caco 2 cell line  (ATCC)
99
ATCC colonic adenocarcinoma caco 2 cell line
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Colonic Adenocarcinoma Caco 2 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cell line colon epithelial cells  (ATCC)
96
ATCC human cell line colon epithelial cells
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Human Cell Line Colon Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ell lines colo320dm  (ATCC)
95
ATCC ell lines colo320dm
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Ell Lines Colo320dm, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human colonic epithelial cells  (ATCC)
97
ATCC human colonic epithelial cells
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Human Colonic Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human recombinant gm-csf  (Schering-Plough corporation)
90
Schering-Plough corporation human recombinant gm-csf
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Human Recombinant Gm Csf, supplied by Schering-Plough corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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easysep human cd4 t cell isolation kit  (STEMCELL Technologies Inc)
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STEMCELL Technologies Inc easysep human cd4 t cell isolation kit
Dependence of Atox1 and p53 level in <t>HCT116</t> and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.
Easysep Human Cd4 T Cell Isolation Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Dependence of Atox1 and p53 level in HCT116 and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.

Journal: bioRxiv

Article Title: The p53 Protein is a Suppressor of Atox1 Copper Chaperon in Tumor Cells Under Genotoxic Effects

doi: 10.1101/2023.07.25.550476

Figure Lengend Snippet: Dependence of Atox1 and p53 level in HCT116 and A549 cell lines with different TP53 status: A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A, and ATOX1 genes; GAPDH gene was used as a reference. C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, paired Student t-test, p < 0,05.

Article Snippet: Transformed human cell lines used: HCT116 (colon adenocarcinoma) with intact p53; HCT116p53 -/- with a deletion of both alleles of the TP53 genes, as well as the A549 line with wild (A549) and knockout p53 (A549p53 -/- ) by the CRISPR-Cas9 method, acquired at ATCC.

Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining

Influence of cytotoxic agents on the activity of Atox1 at different status (WT and KO) of the TP53 gene in A549 and HCT116 cell lines, 24h after drugs exposure. A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A and ATOX1 genes; GAPDH gene was used as a reference, C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. DOX – doxorubicin (0,1μM), CIS – cisplatin (35μM), PMA – phorbol-12-myristate- 13-acetate (80nM), H 2 O 2 – hydrogen peroxide (450μM), BLE – bleomycin (10μM). WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, two-way ANOVA, p < 0,05.

Journal: bioRxiv

Article Title: The p53 Protein is a Suppressor of Atox1 Copper Chaperon in Tumor Cells Under Genotoxic Effects

doi: 10.1101/2023.07.25.550476

Figure Lengend Snippet: Influence of cytotoxic agents on the activity of Atox1 at different status (WT and KO) of the TP53 gene in A549 and HCT116 cell lines, 24h after drugs exposure. A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A and ATOX1 genes; GAPDH gene was used as a reference, C - immunofluorescence staining with primary antibodies to Atox1, secondary antibodies with AlexaFluor488. DAPI was used for nuclei staining. DOX – doxorubicin (0,1μM), CIS – cisplatin (35μM), PMA – phorbol-12-myristate- 13-acetate (80nM), H 2 O 2 – hydrogen peroxide (450μM), BLE – bleomycin (10μM). WT – wild type cells, TP53 -/- – cells without TP53. For all experiments: n = 3, mean +/− SEM, two-way ANOVA, p < 0,05.

Article Snippet: Transformed human cell lines used: HCT116 (colon adenocarcinoma) with intact p53; HCT116p53 -/- with a deletion of both alleles of the TP53 genes, as well as the A549 line with wild (A549) and knockout p53 (A549p53 -/- ) by the CRISPR-Cas9 method, acquired at ATCC.

Techniques: Activity Assay, Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining

Influence of ionizing radiation on the activity of Atox1 at different status (WT and KO) of the TP53 gene in A549 and HCT116 cell lines, 24h after ionizing irradiation (10Gy) exposure. A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A and ATOX1 genes; GAPDH gene was used as a reference. The value of WT 0Gy (control) was taken as a 1.0 for all genes and is not shown in the graphs. For all experiments: n = 3, mean +/− SEM, two-way ANOVA, p < 0,05.

Journal: bioRxiv

Article Title: The p53 Protein is a Suppressor of Atox1 Copper Chaperon in Tumor Cells Under Genotoxic Effects

doi: 10.1101/2023.07.25.550476

Figure Lengend Snippet: Influence of ionizing radiation on the activity of Atox1 at different status (WT and KO) of the TP53 gene in A549 and HCT116 cell lines, 24h after ionizing irradiation (10Gy) exposure. A - immunoblotting with antibodies to p53, p21, and Atox1; beta-actin was used as a normalization. A densitometric analysis of the obtained data is shown below. B – RT-qPCR analysis with primers for TP53, CDKN1A and ATOX1 genes; GAPDH gene was used as a reference. The value of WT 0Gy (control) was taken as a 1.0 for all genes and is not shown in the graphs. For all experiments: n = 3, mean +/− SEM, two-way ANOVA, p < 0,05.

Article Snippet: Transformed human cell lines used: HCT116 (colon adenocarcinoma) with intact p53; HCT116p53 -/- with a deletion of both alleles of the TP53 genes, as well as the A549 line with wild (A549) and knockout p53 (A549p53 -/- ) by the CRISPR-Cas9 method, acquired at ATCC.

Techniques: Activity Assay, Irradiation, Western Blot, Quantitative RT-PCR, Control